Interferon gamma Protein (IFNG)
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- Target See all Interferon gamma (IFNG) Proteins
- Interferon gamma (IFNG)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Characteristics
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ED50<0.05 ng/mL, measured by cytotoxicity assay using HT-29 cells.
Gln24-Gln166 (accession number:P01579). Expression construct with an N-terminal Met. - Purity
- > 95 % as analyzed by reducing SDS-PAGE.
- Endotoxin Level
- < 1 EU/μg, determined by LAL method.
- Top Product
- Discover our top product IFNG Protein
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Interferon gamma (IFNG) protein (His-Avi Tag)
IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interferon gamma (IFNG) protein (His-Avi Tag,Biotin)IFNG Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Interferon gamma (IFNG) (Active) proteinIFNG Origin: Rat Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interferon gamma (IFNG) (AA 23-155) (Active) proteinIFNG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by reducing SDS-PAGE. Active
Interferon gamma (IFNG) proteinIFNG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interferon gamma (IFNG) (AA 24-166) protein (His tag)IFNG Origin: Pig Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 1-144) protein (His tag)IFNG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interferon gamma (IFNG) (AA 24-174) protein (His tag)IFNG Origin: Hamster Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interferon gamma (IFNG) (AA 57-166) protein (GST tag)IFNG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
- Buffer
- Lyophilized after extensive dialysis against PBS.
- Storage
- -80 °C
- Storage Comment
- Lyophilized recombinant human Interferon gamma (rhIFN-γ) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIFN-γ should be stable up to 1 week at 4°C or up to 2 months at -20°C.
- Expiry Date
- 6 months
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- Interferon-gamma (IFN-Gamma) (IFNG Products)
- Background
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Human Interferon gamma (hIFN-γ) is amacrophage-activating factor and the lone member of Interferon type II.The active form of IFN-γ is an antiparallel dimer that interacts with the receptor IFN-γR1 and sets off IFN-γ/JAK/STAT pathway. IFN-γ signaling does diverse biological functions primarily related to host defense and immune regulation, including antiviral and antibacterial defense, apoptosis, inflammation, and innate and acquired immunity. While IFN-γ-induced inflammatory cascade summons a variety of immune-related cell types, such as macrophages, natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), IFN-γ is also implicated in resistance to NK cell and CTL responses and in immune escape in a variety of cancers. Recombinant human Interferon gamma (rhIFN-γ) produced in E. coli is a non-glycosylated polypeptide chain of 144 amino acids. A fully biologically active molecule, rhIFN-γ has a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Synonyms: Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI,IFN-gamma. - Molecular Weight
- 17kDa, observed by reducing SDS-PAGE
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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