IL-18 Protein (AA 37-194)

Catalog No. ABIN2018072

This Recombinant IL-18 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-18 Protein (AA 37-194) (ABIN2018072)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE analyses.

Product Details

Protein Characteristics: AA 37-194

Characteristics: ED50 <2 μg/mL resulted in the secretion of 0.15 ng/mL h-IFN-gamma when tested using KG-1 cell line, corresponding to a specific activity of > 500 units/mg.
AA 37-194, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM Tris, 50 mM NaCl, pH 8.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: Interleukin-18 (IL-18)

Background: Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.Recombinant rat Interleukin-18 (rrIL-18) produced in E. coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Interferon-gamma-inducing factor, IGIF, IL-1g, IL1F4, IL-1 gamma

Molecular Weight: 18.4 kDa, observed by reducing SDS-PAGE.

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome