FGF7 Protein (AA 32-194)

Catalog No. ABIN2018180

Recombinant FGF7 protein expressed in Escherichia coli (E. coli).

Quick Overview for FGF7 Protein (AA 32-194) (ABIN2018180)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Product Details

Protein Characteristics: AA 32-194

Characteristics: ED50 < 2 ng/mL, measured by a cell proliferation assay using 4MBr-5 cells, corresponding to a specific activity of > 5.0x 10^5 units/mg.
AA 32-194, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Keratinocyte Growth Factor (rhKGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhKGF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: Keratinocyte Growth Factor (KGF/FGF-7)

Background: Keratinocyte Growth Factor (KGF) is a highly specific epithelial mitogen produced by fibroblasts and mesenchymal stem cells. KGF belongs to the heparin binding Fibroblast Growth Factor (FGF) family, and is known as FGF-7. However, in contrast to the FGF-1, which binds to all known FGF receptors with high affinity, KGF only binds to a splice variant of an FGF receptor, FGFR2-IIIb. FGFR2-IIIb is produced by most of the epithelial cells, indicating that KGF plays roles as a paracrine mediator. KGF induces the differen-tiation and proliferation of various epithelial cells, including keratinocytes in the epidermis, hair follicles and sebaceous glands, and is responsible for the wound repairs of various tissues, including lung, bladder, and kidney. Recombinant human Keratinocyte Growth Factor (rhKGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 164 amino acids. A fully biologically active molecule, rhKGF has a molecular mass of 19.0 kDa analyzed by reducing SDS-PAGE.
Synonyms: Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7, FGF-7

Molecular Weight: 19.0 kDa, observed by reducing SDS-PAGE.

UniProt: P21781

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway