Oncostatin M Protein (OSM) (AA 26-234)

Catalog No. ABIN2018297

This Recombinant Oncostatin M protein is expressed in Escherichia coli (E. coli).

Quick Overview for Oncostatin M Protein (OSM) (AA 26-234) (ABIN2018297)

Target: Oncostatin M (OSM)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 26-234

Characteristics: ED50 < 10 ng/mL, measured by a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1x 10^5 units/mg.
AA 26-234, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant human Oncostatin M(209 a.a.) (rhOSM) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhOSM should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: Oncostatin M (OSM)

Background: Oncostatin M (OSM) is a multifunctional cytokine, and belongs to Interleukin-6 (IL-6) subfamily, which also includes IL-11, leukemia inhibitory factor (LIF), ciliary neurotropic factor, cardiotrophin-1, and novel neurotropin-1. In vivo, OSM is secreted from activated T cells, monocytes, neutrophils, and endothelial cells. OSM is related to LIF, and shares a receptor with LIF in human. Human OSM can bind to gp130 and recruit OSM Receptor beta or LIF Receptor beta to form a ternary complex. OSM stimulates the growth of different types of cells, including megakaryocytes, fibroblasts, vascular endothelial cells, and T cells. OSM inhibits the proliferation of several cancer cell lines, such as solid tissue tumor cells, lung cancer cells, melanoma cells, and breast cancer cells. Recombinant human Oncostatin M(209 a.a.) (rhOSM) produced in E. coli is a single non-glycosylated polypeptide chain containing 210 amino acids. A fully biologically active molecule, rhOSM has a molecular mass of 23.8 kDa analyzed by reducing SDS-PAGE.
Synonyms: OSM

Molecular Weight: 23.8 kDa, observed by reducing SDS-PAGE.

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion