PDGF-BB Homodimer (AA 82-190) (Active) Protein

Catalog No. ABIN2018322

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for PDGF-BB Homodimer (AA 82-190) (Active) Protein (ABIN2018322)

Target: PDGF-BB Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE analysis.

Product Details

Protein Characteristics: AA 82-190

Characteristics: ED50 < 2.5 ng/mL, measured by a cell proliferation assay using 3T3 Cells, corresponding to a specific activity of > 4 x 10^5 units/mg.
AA 82-190, expressed with an N-terminal Met.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmPDGF-BB remains stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: PDGF-BB Homodimer

Alternative Name: Platelet-Derived Growth Factor-BB (PDGF-BB)

Background: Platelet-Derived Growth Factor-BB (PDGF-BB) is one of five dimers (PDGF-AA, AB, BB, CC, and DD) formed by 4 different PDGF subunits. In vivo PDGF-BB is mainly produced in heart and placenta, and predominantly expressed by osteoblasts, fibroblasts, smooth muscle cells, and glial cells. An inactive precursor of PDGF-BB is produced in the endoplasmic reticulum and then activated by a proprotein convertase after secretion. PDGF-BB functions in a paracrine manner and promotes organogenesis, development of human skeleton, and wound healing. PDGF-BB also promotes angiogenesis, particularly in the presence of Fibroblast Growth Factor basic. Therefore, PDGF-BB and its related pathways are potential pharmacological targets.Recombinant mouse Platelet-Derived Growth Factor-BB (rmPDGF-BB) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 110 amino acids each. A fully biologically active molecule, rmPDGF-BB has a molecular mass of 24.7 kDa analyzed by non-reducing SDS-PAGE.
Synonyms: PDGF-2, GDGF, ODGF, SIS, SSV

Molecular Weight: 24.7 kDa, observed by non-reducing SDS-PAGE.

UniProt: P31240