PDGF-BB Homodimer (AA 82-190) (Active) protein (His tag)

Catalog No. ABIN7197388

This Recombinant PDGF-BB Homodimer protein is expressed in Yeast.

Quick Overview for PDGF-BB Homodimer (AA 82-190) (Active) protein (His tag) (ABIN7197388)

Target: PDGF-BB Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Yeast

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 82-190

Purification tag / Conjugate: His tag

Purpose: Recombinant Human PDGF-BB Protein (His Tag)

Sequence: Ser82-Thr190

Characteristics: A DNA sequence encoding the human PDGFB (NP_148937.1) (Ser82-Thr190) was expressed with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Biological Activity Comment: Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRβ-Fch, the EC₅₀ of human PDGFRβ-Fch is 0.05-0.2 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background: Becaplermin,PDGF Subunit B,PDGF-2,PDGF2,PDGFB,Platelet-Derived Growth Factor B Chain,Platelet-Derived Growth Factor Beta Polypeptide,Platelet-Derived Growth Factor Subunit B,Proto-Oncogene c-Sis,SIS,Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.

Molecular Weight: 14.3 kDa

Gene ID: 5155

NCBI Accession: NP_148937

UniProt: P01127