PDGF-BB Homodimer (AA 82-190) (Active) protein (His tag)

Catalog No. ABIN7320790

Recombinant PDGF-BB Homodimer protein expressed in Escherichia coli (E. coli).

Quick Overview for PDGF-BB Homodimer (AA 82-190) (Active) protein (His tag) (ABIN7320790)

Target: PDGF-BB Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 82-190

Purification tag / Conjugate: His tag

Purpose: Recombinant Mouse PDGF-BB Protein (His Tag)

Sequence: Ser82-Thr190

Characteristics: Recombinant Mouse PDGF-BB is produced by our E.coli expression system and the target gene encoding Ser82-Thr190 is expressed with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using Balb/c3T3 mouse fibroblast cells. The ED₅₀ for this effect is 38.7 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 4 mM HCl.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PDGF-BB Homodimer

Alternative Name: PDGF-BB

Background: Becaplermin,PDGF Subunit B,PDGF-2,PDGF2,PDGFB,Platelet-Derived Growth Factor B Chain,Platelet-Derived Growth Factor Beta Polypeptide,Platelet-Derived Growth Factor Subunit B,Proto-Oncogene c-Sis,SIS,Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. As growth factor,it plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It is required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. PDGFB also plays an important role in wound healing.

Molecular Weight:

Calculated MW: 13.4 kDa

Observed MW: 15 kDa

UniProt: P31240