HBEGF Protein (AA 63-148)

Catalog No. ABIN2018329

Recombinant HBEGF protein expressed in CHO Cells.

Quick Overview for HBEGF Protein (AA 63-148) (ABIN2018329)

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: > 95 % as analyzed by SDS-PAGE and HPLC.

Product Details

Protein Characteristics: AA 63-148

Characteristics: ED50 <0.5 ng/mL, measured in a cell proliferation assay using 3T3 cells.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Storage: -80 °C

Storage Comment: Lyophilized recombinant Human HB-EGF remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, Human HB-EGF should be stable up to 1 week at 4°C or up to 2 months at -20°C.

Expiry Date: 6 months

Target Details

Target: HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))

Alternative Name: Proheparin-Binding EGF-Like Growth Factor (HB-EGF)

Background: Proheparin-binding EGF-like growth factor (HB-EGF), also known as DTR, DTS and HEGFL, is a member of the EGF family of mitogens. It is expressed in macrophages, monocytes, endothelial cells and muscle cells. HB-EGF signals through the EGF receptor to stimulate the proliferation of smooth muscle cells, epithelial cells and keratinocytes. Compared to EGF, HB-EGF binds the EGF receptor with higher affinity and is thus more mitogenic, probably due to its ability to bind to heparin and heparin sulfate proteoglycans. HB-EGF has been reported to act as a diphtheria toxin receptor, mediating endocytosis of the bound toxin.
Synonyms: DTR, DTS, HEGFL

Molecular Weight: 12-14 kDa, observed by reducing SDS-PAGE.

UniProt: Q99075

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway