KIT Ligand Protein (KITLG) (AA 26-189)

Catalog No. ABIN2018380

Product Details

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 26-189

Origin: Mouse

Source: Yeast (Pichia pastoris)

Characteristics: ED50 < 10.0 ng/mL, measured by a cell proliferation assay using human TF-1 cells, corresponding to a specific activity of > 1.0x 10^5 units/mg.
AA 26-189, expressed with an N-terminal Met.

Purity: > 95 % by SDS-PAGE and HPLC analyses.

Endotoxin Level: < 0.2 EU/μg, determined by LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.

Storage: -80 °C

Storage Comment: Lyophilized recombinant mouse Stem Cell Factor (rmSCF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmSCF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Expiry Date: 6 months

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: Stem Cell Factor (SCF) (KITLG Products)

Synonyms: Xkl-1 Protein, Xsl Protein, Xsl-1 Protein, Xsl-2 Protein, steel Protein, KITLG Protein, SCF Protein, kitl Protein, kl-1 Protein, mgf Protein, scf Protein, FPH2 Protein, KL-1 Protein, Kitl Protein, MGF Protein, SF Protein, SHEP7 Protein, Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SLF Protein, Sl Protein, blz Protein, contrasted Protein, 710-712 Protein, CSF Protein, KITL Protein, KIT ligand L homeolog Protein, KIT ligand Protein, kit ligand Protein, kitlg.L Protein, KITLG Protein, kitlg Protein, Kitl Protein, Kitlg Protein

Background: Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant mouse Stem Cell Factor (rmSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rmSCF has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE.
Synonyms: Stem Cell Factor, c-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

Molecular Weight: 18.4kDa, observed by reducing SDS-PAGE.

UniProt: P20826

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway