HMGB1 Protein (AA 2-89)

Catalog No. ABIN2115376

This Recombinant HMGB1 protein is produced in Escherichia coli (E. coli).

Quick Overview for HMGB1 Protein (AA 2-89) (ABIN2115376)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-89

Purpose: Recombinant Human High Mobility Group Protein B1/HMGB1

Sequence: GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKF

Characteristics: Recombinant Human High Mobility Group Protein B1/HMGB1/BOXA is produced by our E. coli expression system. The target protein is expressed with sequence (G2-F89) of Human HMBG1.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 50 mMHepes,500 mMNaCl,0.5 mMDTT, pH 7.9 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High Mobility Group Protein B1/HMGB1

Background: HMGB1 belongs to the HMGB family and contains 2 HMG box DNA-binding domains. HMGB1 is a DNA binding proteins that associates with chromatin and has the ability to bend DNA. This nuclear protein organizes the DNA and regulates transcription. After binding HMGB1 bends the DNA, which conducts to the binding of other proteins. HMGB1 suports transcription of many genes in interactions with many transcription factors or cooperation nucleosoms looses packed DNA and increases the chromatin remodeling, contact with core histones changes the structure of nucleosoms. it involved in V(D)J recombination by acting as a cofactor of the RAG complex. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, stays in the nucleus, but hyperacetylation on lysine residues occurs to translocation into the cytosol.
Synonyms: High-mobility group protein B1, high-mobility group protein 1, HMG-1, amphoterin and HMGB1.

Molecular Weight: 10.5 kDa

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome