C8G Protein (AA 21-202) (His tag)
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- Target See all C8G Proteins
- C8G (Complement Component 8, gamma Polypeptide (C8G))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-202
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This C8G protein is labelled with His tag.
- Purpose
- Recombinant Human Complement Component C8 γ Chain/C8G (N-6His)
- Sequence
- MGSSHHHHHH SSGLVPRGSH MQKPQRPRRP ASPISTIQPK ANFDAQQFAG TWLLVAVGSA CRFLQEQGHR AEATTLHVAP QGTAMAVSTF RKLDGICWQV RQLYGDTGVL GRFLLQARGA RGAVNVVVAE TDYQSFAVLY LERAGQLSVK LYARSLPVSD SVLSGFEQRV QEAHLTEDQI FYFPKYGFCE AADQFHVLDE VRR
- Characteristics
- Recombinant Human C8G is produced with our E. coli expression system. The target protein is expressed with sequence (Gln21-Arg202) of Human C8G fused with a polyhistidine tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product C8G Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- C8G (Complement Component 8, gamma Polypeptide (C8G))
- Alternative Name
- Complement Component C8 gamma Chain (C8G Products)
- Synonyms
- C8C Protein, complement C8 gamma chain Protein, complement component 8, gamma polypeptide Protein, C8G Protein, C8g Protein
- Background
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Complement component C8 is a constituent of the membrane attack complex, C8 alpha, C8 beta and C8G. C8G is a secreted protein and comsists a disulfide-linked C8 alpha-gamma heterodimer and a non-covalently associated C8 beta chain. C8 alpha and C8 beta play an important role in complement-mediated bacterial killing together.C8 is involved in the formation of Membrane Attack Complex on bacterial cell membranes. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. Patients lacking C8 are susceptible to certain bacterial infections.
Synonyms: Complement component C8 gamma chain,C8G - Molecular Weight
- 22.6kDa
- UniProt
- P07360
- Pathways
- Complement System
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