Haptoglobin Protein (HP) (AA 145-406) (His tag)

Catalog No. ABIN2115542

This Recombinant Haptoglobin protein is produced in Escherichia coli (E. coli).

Quick Overview for Haptoglobin Protein (HP) (AA 145-406) (His tag) (ABIN2115542)

Target: Haptoglobin (HP)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 145-406

Purification tag / Conjugate: This Haptoglobin protein is labelled with His tag.

Purpose: Recombinant Human Haptoglobin/Zonulin (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MCEAVCGKPK NPANPVQRIL GGHLDAKGSF PWQAKMVSHH NLTTGATLIN EQWLLTTAKN LFLNHSENAT AKDIAPTLTL YVGKKQLVEI EKVVLHPNYS QVDIGLIKLK QKVSVNERVM PICLPSKDYA EVGRVGYVSG WGRNANFKFT DHLKYVMLPV ADQDQCIRHY EGSTVPEKKT PKSPVGVQPI LNEHTFCAGM SKYQEDTCYG DAGSAFAVHD LEEDTWYATG ILSFDKSCAV AEYGVYVKVT SIQDWVQKTI AEN

Characteristics: Recombinant Human Haptoglobin is produced by our E.coli expression system in human cells. The target protein is expressed with sequence (Cys145-Asn406) of Human Haptoglobin fused with a polyhistidine tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS,1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 6 months

Target Details

Target: Haptoglobin (HP)

Alternative Name: Haptoglobin

Background: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial, Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response.
Synonyms: Haptoglobin,Zonulin,HP

Molecular Weight: 31.4 kDa

UniProt: P00738

Pathways: Transition Metal Ion Homeostasis