Haptoglobin Protein (HP) (AA 19-406) (His tag)

Catalog No. ABIN1945383

Product Details

Target: Haptoglobin (HP)

Protein Type: Recombinant

Protein Characteristics: AA 19-406

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This Haptoglobin protein is labelled with His tag.

Purpose: Recombinant Human Haptoglobin/Zonulin (C-6His)

Sequence: VDSGNDVTDI ADDGCPKPPE IAHGYVEHSV RYQCKNYYKL RTEGDGVYTL NDKKQWINKA VGDKLPECEA DDGCPKPPEI AHGYVEHSVR YQCKNYYKLR TEGDGVYTLN NEKQWINKAV GDKLPECEAV CGKPKNPANP VQ&ILGGHLDAKG SFPWQAKMVS HHNLTTGATL INEQWLLTTA KNLFLNHSEN ATAKDIAPTL TLYVGKKQLV EIEKVVLHPN YSQVDIGLIK LKQKVSVNER VMPICLPSKD YAEVGRVGYV SGWGRNANFK FTDHLKYVML PVADQDQCIR HYEGSTVPEK KTPKSPVGVQ PILNEHTFCA GMSKYQEDTC YGDAGSAFAV HDLEEDTWYA TGILSFDKSC AVAEYGVYVK VTSIQDWVQK TIAENVDHHH HHH

Characteristics: Recombinant Human Haptoglobin is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (AA 19-406) of Human Haptoglobin fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 1 mM DTT, pH 7.4.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Haptoglobin (HP)

Alternative Name: Haptoglobin/Zonulin (HP Products)

Synonyms: HP Protein, wu:fb64e01 Protein, BP Protein, HP2ALPHA2 Protein, HPA1S Protein, HP-1 Protein, HPR Protein, Zonulin Protein, haptoglobin Protein, haptoglobin-like Protein, HP Protein, hp Protein, Hp Protein, LOC479668 Protein, LOC101102413 Protein

Background: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial, Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response.

Molecular Weight: 15.9&28.3 kDa

UniProt: P00738

Pathways: Transition Metal Ion Homeostasis