EGF Protein
Quick Overview for EGF Protein (ABIN2129047)
Target
See all EGF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
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Characteristics
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Purified recombinant Mouse EGF protein
Expression System: E.coli
Bioactivity: The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 10^7 units/mg. -
Endotoxin Level
- < 0.1 ng per μg (1 EU/μg).
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Human Host: Escherichia coli (E. coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, AcA Active
Epidermal Growth Factor (EGF) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Comment
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Biological activity: The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 10^7 units/mg.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Lyophilized from 10 mM Na3PO4, pH 7.5.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- EGF (Epidermal Growth Factor (EGF))
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Alternative Name
- EGF
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Background
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Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF alpha and VGF (vaccinia virus growth factor).
Alternative Names: Epidermal Growth Factor protein, URG protein, Urogastrone protein -
Molecular Weight
- 6.0 kDa
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Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Target
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