FGF2 Protein

Catalog No. ABIN2129215

This Recombinant FGF2 protein is produced in Escherichia coli (E. coli).

Quick Overview for FGF2 Protein (ABIN2129215)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % pure

Product Details

Sequence: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Characteristics: Purified recombinant Human FGF basic protein
Expression System: E.coli
Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ? 0.5 ng/mL, corresponding to a specific activity of ? 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 0.1 ng/mL, corresponding to a specific activity of ? 1 x 10^7 units/mg.

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is approx. 0.5 ng/mL, corresponding to a specific activity of approx. 2 x 10^6 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is approx. 0.1 ng/mL, corresponding to a specific activity of approx. 1 x 10^7 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1 - 1.0 mg/mL.

Buffer: Lyophilized from 5 mM Tris, pH 7.6 with 155 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: FGF Basic

Background: FGF basic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF basic is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary, kidney, retina, bone, testis, adrenal gland liver, monocytes, epithelial cells and endothelial cells. FGF basic signals through FGFR 1b, 1c, 2c, 3c and 4.
Alternative Names: FGF-2 protein, Prostatropin protein, Fibroblast Growth Factor-basic protein, HBGF-2 protein

Molecular Weight: 17.2 kDa

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins