CTLA4 Protein (AA 37-160) (Fc Tag)

Catalog No. ABIN2180932

Recombinant CTLA4 protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for CTLA4 Protein (AA 37-160) (Fc Tag) (ABIN2180932)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >98 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 37-160

Purification tag / Conjugate: This CTLA4 protein is labelled with Fc Tag.

Sequence: AA 37-160

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. The predicted N-terminus is Ala 37. As a result of glycosylation, the reducing (R) protein migrates as 50-55 kDa, and the non-reducing (NR) protein migrates as 100-110 kDa in SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Tris with Glycine, Arginine and NaCl,  pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Gonzalez-Nieto, Castro, Bischof, Shin, Ricciardi, Bailey, Dang, Pedreño-Lopez, Magnani, Ejima, Allison, Gil, Evans, Rakasz, Lifson, Desrosiers, Martins: "Vaccine protection against rectal acquisition of SIVmac239 in rhesus macaques." in: PLoS pathogens, Vol. 15, Issue 9, pp. e1008015, (2019) (PubMed).

Target Details

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Molecular Weight: 39.9 kDa

NCBI Accession: NP_005205

Pathways: Cancer Immune Checkpoints