CTLA4 Protein (AA 36-161) (Biotin,His-Avi Tag)

Catalog No. ABIN7274437

This Recombinant CTLA4 protein is expressed in HEK-293 Cells.

Quick Overview for CTLA4 Protein (AA 36-161) (Biotin,His-Avi Tag) (ABIN7274437)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 36-161

Purification tag / Conjugate: This CTLA4 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CTLA-4/CD152 Protein

Sequence: Lys36-Asp161

Characteristics: Recombinant Biotinylated Human CTLA-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Lys36-Asp161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CTLA-4, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human B7-1, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test).,Immobilized Anti-CTLA-4 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CTLA-4, His Tag with the EC50 of 45.7ng/ml determined by ELISA.,Biotinylated Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CTLA-4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA-4

Background: CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation - a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

Molecular Weight: 16.3 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

UniProt: P16410

Pathways: Cancer Immune Checkpoints