TNF alpha Protein (AA 77-233)

Catalog No. ABIN2181831

Recombinant TNF alpha protein expressed in HEK-293 Cells and has been mentioned in 3+ publications.

Quick Overview for TNF alpha Protein (AA 77-233) (ABIN2181831)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Grade: HPLC verified

Product Details

Protein Characteristics: AA 77-233

Brand: ActiveMax®

Sequence: AA 77-233

Characteristics: This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa under reducing (R) condition (SDS-PAGE).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C in lyophilized state after receipt. For long term storage, upon reconstitution rhTNFa should be aliquot and store at -20°C or -80°C.

References

Hua, Oh, Kim, Antonio, Vo, Om, Choi, Kim, Jung, Park, Jeong: "Peroxisome proliferator-activated receptor gamma as a theragnostic target for mesenchymal-type glioblastoma patients." in: Experimental & molecular medicine, Vol. 52, Issue 4, pp. 629-642, (2020) (PubMed).

Yu, Xu, Qu, Yu, Li, Zhao, Qiu: "Decrease of MiR-31 induced by TNF-α inhibitor activates SATB2/RUNX2 pathway and promotes osteogenic differentiation in ethanol-induced osteonecrosis." in: Journal of cellular physiology, Vol. 234, Issue 4, pp. 4314-4326, (2019) (PubMed).

Zhao, Jiang, Wang, Wu: "Overexpression of microRNA-590-3p promotes the proliferation of and inhibits the apoptosis of myocardial cells through inhibition of the NF-κB signaling pathway by binding to RIPK1." in: Journal of cellular biochemistry, Vol. 120, Issue 3, pp. 3559-3573, (2019) (PubMed).

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.

Molecular Weight: 17.4 kDa

NCBI Accession: NP_000585

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome