E-cadherin Protein (AA 23-621) (Fc Tag)

Catalog No. ABIN2444102

This Recombinant E-cadherin protein is produced in HEK-293 Cells. There are 3 publications available.

Quick Overview for E-cadherin Protein (AA 23-621) (Fc Tag) (ABIN2444102)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 23-621

Purification tag / Conjugate: This E-cadherin protein is labelled with Fc Tag.

Sequence: AA 23-621

Characteristics: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 92.4 kDa. As a result of different glycosylation, the protein migrates as 110-125 kDa Cadherin, a 48 kDa Propeptide under reducing (R) condition, and 200-250 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 100 mM Glycine, pH 7.5

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Lyophilized Protein should be stored at -20 °C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20 °C or -70 °C. Avoid repeated freeze-thaw cycles.

References

Eshghi, Gaultney, England, Brûlé, Miras, Sato, Coburn, Bellalou, Moriarty, Haouz, Picardeau: "An extracellular Leptospira interrogans leucine-rich repeat protein binds human E- and VE-cadherins." in: Cellular microbiology, Vol. 21, Issue 2, pp. e12949, (2019) (PubMed).

Campbell, Salvi, OBrien, Superfine, DeMali: "PAK2 links cell survival to mechanotransduction and metabolism." in: The Journal of cell biology, Vol. 218, Issue 6, pp. 1958-1971, (2019) (PubMed).

Suffoletto, Jetta, Hua: "E-cadherin mediated lateral interactions between neighbor cells necessary for collective migration." in: Journal of biomechanics, Vol. 71, pp. 159-166, (2019) (PubMed).

Target Details

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E-Cadherin

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Cadherin-1 (CDH1) is also known as epithelial cadherin (E-cadherin), CD_antigen (CD324), Uvomorulin (UVO) ECAD and CDHE, CDH1 / CD324 contains 5 cadherin domains. CDH1 / CD324 / ECAD is expressed in non-neural epithelial tissues. CDH1 / E-CAD is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells and has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad promotes non-amyloidogenic degradation of Abeta precursors and has a strong inhibitory effect on APP C99 and C83 production. Defects in CDH1 / CD324 / ECAD are the cause of hereditary diffuse gastric cancer (HDGC).

Molecular Weight: 92.3 kDa

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation