E-cadherin Protein (His tag)

Catalog No. ABIN7320196

Product Details

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This E-cadherin protein is labelled with His tag.

Purpose: Recombinant Mouse E-Cadherin/CDH1 Protein (His Tag)(Active)

Sequence: Met 1-Val 709

Characteristics: A DNA sequence encoding the extracellular domain of mouse CDH1 (P09803) (Met 1-Val 709) was expressed, with a C-terminal polyhistidine tag.

Purity: > 85 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When 5 x 10E4 cells/well are added to Recombinant Mouse Cadherin-1 coated plates (0.8 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E-Cadherin/CDH1 (CDH1 Products)

Synonyms: Arc-1 Protein, CD324 Protein, CDHE Protein, ECAD Protein, LCAM Protein, UVO Protein, E-cadherin Protein, cdh1 Protein, si:dz180o5.2 Protein, uvo Protein, lcam Protein, E-Cad Protein, XBcad Protein, l-cam Protein, xcdh1 Protein, cdhc-A Protein, xb-cad Protein, XTCAD-1 Protein, c-cadherin Protein, cadherin-1 Protein, uvomorulin Protein, XB-cadherin Protein, arc-1 Protein, cd324 Protein, cdhe Protein, ecad Protein, AA960649 Protein, E-cad Protein, Ecad Protein, L-CAM Protein, Um Protein, cadherin 1 Protein, si:busm1-180o5.2 Protein, cadherin 1, type 1, E-cadherin (epithelial) Protein, cadherin 1, type 1 S homeolog Protein, CDH1 Protein, si:busm1-180o5.2 Protein, cdh1 Protein, Cdh1 Protein, cdh1.S Protein

Background:

Background: Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Synonym: AA960649,ARC-1,E-cad,Ecad,L-CAM,Um,UVO

Molecular Weight: 78 kDa

UniProt: P09803

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation