RuvC Protein
Native RuvC protein expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.
(3 references)Quick Overview for RuvC Protein (ABIN2452186)
Target
RuvC
(Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
Protein Type
Native
Biological Activity
Active
Origin
E. coli
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
-
-
Characteristics
- Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
-
-
Want other Options for this Protein ?
!Discover Our Predefined Custom Proteins and Custom Protein Services!ProductExpression SystemConjugateOriginPrice starts atExpression System RabbitConjugateOrigin DiscosomaPrice starts at $350.00Expression System Guinea PigConjugateOrigin RabbitPrice starts at $350.00Expression System RabbitConjugateOrigin Cow, Human, Mouse, Pig, Rabbit, RatPrice starts at $129.85Expression System RabbitConjugateOrigin Cow, Human, Mouse, Pig, RatPrice starts at $414.72
$460.80-10%Expression System GoatConjugateOrigin Aequorea victoriaPrice starts at $350.00Expression SystemConjugateOrigin RatPrice starts at $588.00Expression System RabbitConjugateOrigin HumanPrice starts at $637.78Expression System MouseConjugateOrigin HumanPrice starts at $651.43Expression System RabbitConjugateOrigin Human, Monkey, Mouse, Pig, Rat, SheepPrice starts at $384.62Expression System RabbitConjugateOrigin Horse, Human, Mouse, Rabbit, RatPrice starts at $384.62Your project requires further customization? Contact us and discover our custom protein solutions
-
Crossover Junction Endodeoxyribonuclease RuvC (RuvC) (AA 2-173) protein (His-SUMO Tag)
RuvC Origin: E. coli Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
-
-
Application Notes
-
1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA -
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1.0 mg/mL
-
Buffer
- 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
-
Storage
- -20 °C/-80 °C
-
Storage Comment
- Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
-
-
-
: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).
: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).
-
: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).
-
- RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
-
Alternative Name
- RuvC
-
Background
- E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
-
UniProt
- P0A814
Target
-
You are here:
