Angiopoietin 2 Protein (ANGPT2)

Catalog No. ABIN2468225

This Recombinant Angiopoietin 2 protein is produced in CHO Cells.

Quick Overview for Angiopoietin 2 Protein (ANGPT2) (ABIN2468225)

Target: Angiopoietin 2 (ANGPT2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: < 95 % by SDS-PAGE gel and HPLC analyses.

Product Details

Sequence: DAPLEYDDSV QRLQVLENIM ENNTQWLMKL ENYIQDNMKK EMVEIQQNAV QNQTAVMIEI GTNLLNQTAE QTRKLTDVEA QVLNQTTRLE LQLLEHSLST NKLEKQILDQ TSEINKLQDK NSFLEKKVLA MEDKHIIQLQ SIKEEKDQLQ VLVSKQNSII EELEKKIVTA TVNNSVLQKQ QHDLMETVNN LLTMMSTSNS AKDPTVAKEE QISFRDCAEV FKSGHTTNGI YTLTFPNSTE EIKAYCDMEA GGGGWTIIQR REDGSVDFQR TWKEYKVGFG NPSGEYWLGN EFVSQLTNQQ RYVLKIHLKD WEGNEAYSLY EHFYLSSEEL NYRIHLKGLT GTAGKISSIS QPGNDFSTKD GDNDKCICKC SQMLTGGWWF DACGPSNLNG MYYPQRQNTN KFNGIKWYYW KGSGYSLKAT TMMIRPADFH HHHHH

Characteristics: Biological activity was determined by its ability to stimulate tubulogenesis in HUVEC cells using a concentration of 0.2 μg/mL.

Endotoxin Level: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Handling Advice: As with any protein, exposing Yeast ANG-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -20 °C

Storage Comment: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted ANG-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Expiry Date: 24 months

Target Details

Target: Angiopoietin 2 (ANGPT2)

Alternative Name: ANG-2

Background: ANG-2 binds to the endothelial cell specific receptor Tie2, but, in contrast to ANG-1 does not induce tyrosine phosphorylation. Consequently, ANG-2 modulates ANG-1 activation of Tie2 and, depending on the physiological and biochemical environment, can act either as a n agonist or antagonist of Tie2 induced angiogenesis. The signaling interactions of ANG-1, ANG-2 and Tie2, along with less characterized ANG-3 and ANG-4, are required for embryonic and adult angiogenesis. Physiologically, ANG-1 and ANG-2 are associated with sprouting, tube formation, and structural integrity of newly formed blood vessels. Mature human ANG-2 is a secreted protein containing 480 amino acid residues. ANG-2 is composed of an alpha helix rich "coiled coil N-terminal domain and fibrinogen like C-terminal domain. ANG-2 exists predominantly in the form of a disulfide-linked dimer. Recombinant human ANG-2 is a C-terminal histidine tagged glycoprotein which migrates with an apparent molecular mass of 60.0 - 70.0 kDa by SDS-PAGE under reducing conditions. Sequencing analysis shows an N-terminal sequence starting with residue 68 (D) of the ANG-2 precursor protein.

Gene ID: 285

NCBI Accession: NP_001112359

OMIM: 169646749

UniProt: O15123

Pathways: RTK Signaling