PUMA Protein (AA 76-170) (His tag,Strep II tag)
Quick Overview for PUMA Protein (AA 76-170) (His tag,Strep II tag) (ABIN2468998)
Target
See all PUMA (BBC3) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 76-170
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Purification tag / Conjugate
- This PUMA protein is labelled with His tag,Strep II tag.
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BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (His tag)
custom-made BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 1-261) protein (His tag)custom-made BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 1-193) protein (His tag)custom-made BBC3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2 Binding Component 3 (BBC3) (AA 141-261) protein (His-IF2DI Tag)BBC3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
BCL2 Binding Component 3 (BBC3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)BBC3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- This recombinant protein can be used for WB, ELISA, MS.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- 1X PBS containing 0.1 % SDS and 0.02 % NaN
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Storage
- -80 °C
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Storage Comment
- Store in working aliquots at -70 °C.
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- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Alternative Name
- PUMA
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Background
- Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-? and PUMA-? (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis. This PUMA recombinant protein is recognized by several ProSci PUMA monoclonal antibodies, but not the two polyclonal antibodies (PSI # 3041 and 3043).
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Molecular Weight
- 15 kDa (Calculated)
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Gene ID
- 27113
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OMIM
- 56748610
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UniProt
- Q9BXH1
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Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
Target
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