VEGF 165 (Active) Protein
Quick Overview for VEGF 165 (Active) Protein (ABIN2469210)
Target
Protein Type
Biological Activity
Origin
Source
Purity
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Sequence
- MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
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Characteristics
- Biological activity was determined by the dose - dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0 - 8.0 ng/mL.
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Endotoxin Level
- Endotoxin level is less than 0.1 ng per μg (1 EU/μg).
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VEGF 165 (AA 27-191) (Active) protein
Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
VEGF 165 (AA 27-191) (Active) protein (Biotin,His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
VEGF 165 (AA 27-191) (Active) protein (His tag,AVI tag,Biotin)Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
VEGF 165 (AA 27-191) (Active) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
VEGF 165 proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
VEGF 165 (AA 207-371) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
VEGF 165 (AA 27-191) protein (His tag)Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
VEGF 165 (AA 207-371) proteinOrigin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Handling Advice
- As with any protein, exposing VEGF165 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Storage
- -20 °C
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Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted VEGF165 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
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Expiry Date
- 24 months
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- VEGF 165
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Alternative Name
- VEGF165
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Background
- VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothellial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors , fms-like tyrosine kinase(flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant rat VEGF is a 38.5 kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.
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Gene ID
- 83785
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NCBI Accession
- NP_001103803
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OMIM
- 160358793
Target
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