IL-6 Receptor Protein (His tag)

Catalog No. ABIN2566357

This Recombinant IL-6 Receptor protein is expressed in HEK-293 Cells.

Quick Overview for IL-6 Receptor Protein (His tag) (ABIN2566357)

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB), ELISA

Purity: >92 % as determined by SDS-PAGE.

Product Details

Purification tag / Conjugate: This IL-6 Receptor protein is labelled with His tag.

Characteristics:

Measured by its binding ability in a functional ELISA. Immobilized Human IL6R /CD126 Protein at 5 μg/mL (100 μL/well),can bind Biotinylated rh IL6 Protein. The EC50 is typically 0. 06-0. 6 μg/mL. Measured by its ability to enhance the IL6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 15-60 ng/mL.

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA. For research use only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -80 °C,-20 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: IL-6 Receptor (IL6R) (Interleukin 6 Receptor (IL6R))

Alternative Name: CD126

Background: Interleukin 6 receptor (IL6R) is also known as CD126 (Cluster of Differentiation 126) , is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL6R is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit also shared by many other cytokines. The soluble form of IL6R arises from proteolytic cleavage of membrane-bound IL6Rα, and acts agonistically by making the IL6 ligand accessible to the signal transducer gp130. Dysregulated production of IL6 and IL6R are implicated in the pathogenesis of several inflammatory diseases and malignancies such as multiple myeloma, rheumatoid arthritis, or osteoporosis, and it has been reported that a humanized anti-IL6R monoclonal antibody is a promising agent applicable to the therapeutic approach for IL6 driven diseases. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor.

Molecular Weight: 40 kDa

Gene ID: 3570

NCBI Accession: NP_000556

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints