RSPO1 Protein (AA 21-146) (His tag)

Catalog No. ABIN2566884

This Recombinant RSPO1 protein is produced in HEK-293 Cells.

Quick Overview for RSPO1 Protein (AA 21-146) (His tag) (ABIN2566884)

Target: RSPO1 (R-Spondin 1 (RSPO1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB), ELISA

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 21-146

Purification tag / Conjugate: This RSPO1 protein is labelled with His tag.

Characteristics:

Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/mL (100 μL/well) can bind human LIMPII with a linear ranger of 12. 5 - 1000 ng/mL.

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA. For research use only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -80 °C,-20 °C

Storage Comment: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Target Details

Target: RSPO1 (R-Spondin 1 (RSPO1))

Alternative Name: R Spondin 1 ()

Background: R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively. R-Spondin-1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development. Human RSPO1 disruption results in a recessive syndrome characterized by XX sex reversal, palmoplantar hyperkeratosis and predisposition to squamous cell carcinoma of the skin. It has been shown that the complete female-to-male sex reversal is due to the absence of the testis-determining gene, SRY. R-Spondin-1 regulates Wnt/β-catenin by competing with the Wnt antagonist DKK1 for binding to the Wnt co receptors, Kremen and LRP6, reducing their DKK1 mediated internalization. Reports differ on whether R-spondin 1 binds LRP6 directly.

Molecular Weight: 16.7 kDa

Gene ID: 284654

UniProt: Q2MKA7