Placenta Growth Factor 1 (PIGF-1) (AA 21-149) (Active) Protein

Catalog No. ABIN2666440

This Recombinant Placenta Growth Factor 1 protein is produced in HEK-293 Cells.

Quick Overview for Placenta Growth Factor 1 (PIGF-1) (AA 21-149) (Active) Protein (ABIN2666440)

Target: Placenta Growth Factor 1 (PIGF-1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Biochemical Assay (BCA)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 21-149

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 = 1-3 ng/ml, corresponding to a specific activity of 0.33 - 1 x 106 units/mg, as determined by the dose dependent binding to VEGFR1-Fc chimera using a functional ELISA.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 5 mM citric acid, 0.15 M NaCl, 5 mM NaHPO4, pH 4.0.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: Placenta Growth Factor 1 (PIGF-1)

Alternative Name: PLGF-1

Background: Placenta growth factor (PLGF) was initially cloned form a term placenta cDNA library. PLGF is a dimeric glycoprotein with 53 % homology to VEGF. As a result of alternative splicing, four isoforms for PLGF has been described, PLGF-1, PLGF-2, PLGF-3, and PLGF-4. PLGF-2 binds to heparin with high affinity, whereas neither PLGF-1, PLGF-3, nor PLGF-4 bind heparin. PLGF-2 is the only isoform in mouse. PLGF binds to VEGFRI but not to VEGFRII, also PLGF binds to sVEGF1. PLGF plays a key role in the maternal vascular function during pregnancy. Low levels of PLGF have been associated with patients experiencing preeclampsia and delivering small-for-gestational age (SGA) neonates. High expression of PLGF has been associated to colorectal cancer (CRC), and its expression in CRC tissues correlates with high levels of MMP-9 and VEGF1. PLGF has been shown to increase tumor cell migration in lung cancer, leukemia, and melanoma cells. In colorectal cancer, PLGF induces tumor cell migration and invasion through upregulation of MMP9. PLGF is expressed in the majority of medulloblastomas, and high expression of PLGF receptor neuropilin 1 (Nrp1) correlates with poor survival in patients. In a mouse model, PLGF/Nrp1 blockade provided direct antitumor effects in vivo, medulloblastoma regression, decreased metastasis, and increased survival.

Molecular Weight: The 129 amino acid recombinant protein has a predicted molecular mass of approximately 14.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 20-25 kDa and 45-50 kDa by SDS-PAGE respectively. The N-terminal amino acid is Ala.