Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CCL1 Protein (AA 24-92)

CCL1 Origin: Mouse Host: HEK-293 Cells Recombinant > 98 % , as determined by Coomassie stained SDS-PAGE. WB Active
Catalog No. ABIN2666951
  • Target See all CCL1 Proteins
    CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 24-92
    Origin
    • 12
    • 3
    • 1
    • 1
    Mouse
    Source
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Application
    Western Blotting (WB)
    Purity
    > 98 % , as determined by Coomassie stained SDS-PAGE.
    Sterility
    0.22 μm filtered
    Endotoxin Level

    Less than 0.01 ng per μg cytokine as determined by the LAL method.

    Top Product
    Discover our top product CCL1 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological activity: Bioactivity was measured by its property to chemoattract mouse BW.5147.G.1.4OUAR.1 cells in a dose dependent manner.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA, or HAS. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
    Buffer
    0.22 μm filtered protein solution is in PBS.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    -20 °C
    Storage Comment
    Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
  • Target
    CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
    Alternative Name
    CCL1 (CCL1 Products)
    Synonyms
    I-309 Protein, P500 Protein, SCYA1 Protein, SISe Protein, TCA3 Protein, BF534335 Protein, Scya1 Protein, Tca-3 Protein, CCLi6 Protein, chemokine Protein, C-C motif chemokine ligand 1 Protein, chemokine (C-C motif) ligand 1 Protein, CCL1 Protein, Ccl1 Protein
    Background
    CCL1 was initially identified as a secreted protein derived from activated T cells. CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation, as a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatics patients compared with normal controls. In addition, high levels of CCL1 have been detected in serum of patients with atopic dermatitis. Also, CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.
    Molecular Weight
    The 69 amino acid recombinant protein has a predicted molecular mass of approximately 7.7 kDa. The DTT-reduced protein migrates at approximately 16 kDa and non-reduced protein migrates at approximately 18-20 kDa by SDS-PAGE. The N-terminal amino acid is L
You are here:
Support