IL-6 Protein (AA 25-211)

Catalog No. ABIN2667538

This Recombinant IL-6 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-6 Protein (AA 25-211) (ABIN2667538)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Multiplex Assay (MA)

Purity: Purity is >98 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 25-211

Sterility: 0.22 μm filtered

Endotoxin Level:

Endotoxin level is <0.1 EU/μg (<0.01ng/μg) protein as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: The ED50 is < 0.01 ng/ml, corresponding to a specific activity of > 1 x 108 units/mg, as determined by the dose dependent stimulation of 7TD1 cell proliferation.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution in Sodium Acetate and EDTA.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (-/-) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture (1). The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate chain (IL-6R), and a ubiquitously expressed 130-kD β -chain receptor (gp130) which acts as the universal signal-transducing element for all IL-6 family cytokines (2). Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling) (3). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation, as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production (4). IL-6 is a key factor that reciprocally regulates Th17 and Foxp3(+) Treg differentiation by inhibition of TGF-beta induced Foxp3 and induction of RORgammat, a Th17 lineage-specific transcription factor (5).

Molecular Weight: The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,866 Da. The DTT-reduced protein migrates at approximately 22 kDa and the non-reduced protein migrates at approximately 21.5 kDa by SDS-PAGE.

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome