Quick Overview for CSTA Protein (Myc-DYKDDDDK Tag) (ABIN2712791)
Target
See all CSTA ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This CSTA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Cystatin-A protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (His tag)
custom-made CSTA Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-97) protein (His tag)custom-made CSTA Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (GST tag)CSTA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cystatin A (Stefin A) (CSTA) (AA 1-98) protein (His-IF2DI Tag)CSTA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Cystatin A (Stefin A) (CSTA) (AA 1-98) (Active) protein (His tag)CSTA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, EAA Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- CSTA (Cystatin A (Stefin A) (CSTA))
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Alternative Name
- Cystatin-A
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Background
- The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins, and kininogens. This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and the cathepsins B, H, and L. The protein is one of the precursor proteins of cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Stefins have been proposed as prognostic and diagnostic tools for cancer.
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Molecular Weight
- 10.8 kDa
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NCBI Accession
- NP_005204
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Pathways
- Response to Water Deprivation
Target
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