CTLA4 Protein (Transcript Variant 1) (Fc Tag)

Catalog No. ABIN2713438

This Recombinant CTLA4 protein is expressed in CHO Cells.

Quick Overview for CTLA4 Protein (Transcript Variant 1) (Fc Tag) (ABIN2713438)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Application: Antibody Production (AbP), Standard (STD), Functional Studies (Func), Protein Interaction (PI)

Purity: > 95 % , as determined by Coomassie stained SDS-PAGE

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This CTLA4 protein is labelled with Fc Tag.

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human CD152 / CTLA4 (transcript variant 1) protein expressed in CHO cells.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Endotoxin Level: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 75 - 375 ng/ml, corresponding to a specific activity of 0.2-1.3 x 104 units/mg, determined by the dose dependent inhibition of IL-2 production induced by 0.5 μg/ml of CD80 on Jurkat cells.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Concentration: > 50 μg/mL

Buffer: 1 x PBS

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: Cd152,ctla4

Background: This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.

Molecular Weight: 39.9 kDa

NCBI Accession: NP_005205

Pathways: Cancer Immune Checkpoints