AATF Protein (DYKDDDDK Tag)

Catalog No. ABIN2714258

Recombinant AATF protein expressed in Insect cells (Sf9).

Quick Overview for AATF Protein (DYKDDDDK Tag) (ABIN2714258)

Target: AATF (Apoptosis Antagonizing Transcription Factor (AATF))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This AATF protein is labelled with DYKDDDDK Tag.

Characteristics:

• Recombinant human AATF (full length, C-term DDK tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: AATF (Apoptosis Antagonizing Transcription Factor (AATF))

Alternative Name: Aatf

Background: The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

Molecular Weight: 63 kDa

NCBI Accession: NP_036270

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome