ALT Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for ALT Protein (Myc-DYKDDDDK Tag) (ABIN2714525)
Target
See all ALT ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ALT protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Alanine aminotransferase 1 / ALT1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Alanine Aminotransferase (ALT) protein (His tag)
ALT Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Alanine Aminotransferase (ALT) (AA 1-496) protein (GST tag)ALT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Alanine Aminotransferase (ALT) (AA 1-496) protein (His tag)ALT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) (Active) protein (His tag)ALT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90% by SDS-PAGE SDS, EAA Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
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: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
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- ALT (Alanine Aminotransferase (ALT))
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Alternative Name
- Alanine Aminotransferase 1 (Alt1)
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Background
- This gene encodes cytosolic alanine aminotransaminase 1 (ALT1) also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009].
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Molecular Weight
- 54.5 kDa
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NCBI Accession
- NP_005300
Target
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