ALT Protein (AA 1-496) (His tag)
Quick Overview for ALT Protein (AA 1-496) (His tag) (ABIN667978)
Target
See all ALT ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-496
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Purification tag / Conjugate
- This ALT protein is labelled with His tag.
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Sequence
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MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS -
Characteristics
- GPT, 1-496aa , Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Alanine Aminotransferase (ALT) protein (His tag)
ALT Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Alanine Aminotransferase (ALT) (AA 1-496) protein (GST tag)ALT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)ALT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)ALT Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) (Active) protein (His tag)ALT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS, EAA Active
Alanine Aminotransferase (ALT) protein (Myc-DYKDDDDK Tag)ALT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 2 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ALT (Alanine Aminotransferase (ALT))
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Alternative Name
- ALT1/GPT1
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Background
- GPT, also known as alanine aminotransferases (ALT1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant human GPT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 56.8 kDa (516aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_005300
Target
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