ALT Protein (AA 1-496) (His tag)
Quick Overview for ALT Protein (AA 1-496) (His tag) (ABIN6387974)
Target
See all ALT ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-496
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Purification tag / Conjugate
- This ALT protein is labelled with His tag.
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Sequence
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MASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS -
Purification
- > 90% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 100unit/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
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Alanine Aminotransferase (ALT) protein (His tag)
ALT Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Alanine Aminotransferase (ALT) (AA 1-496) protein (GST tag)ALT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)ALT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) protein (His-IF2DI Tag)ALT Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Alanine Aminotransferase (ALT) (AA 1-496) protein (His tag)ALT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Alanine Aminotransferase (ALT) protein (Myc-DYKDDDDK Tag)ALT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Bioactivity Validated
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Phosphate-Buffered Saline ( pH 7.4) containing 1 mM DTT, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ALT (Alanine Aminotransferase (ALT))
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Alternative Name
- ALT1/GPT1
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Background
- Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant mouse Gpt protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 57.5 kDa (519aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_877957
Target
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