ALT Protein (Myc-DYKDDDDK Tag)
(1 reference)-
- Target See all ALT Proteins
- ALT (Alanine Aminotransferase (ALT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ALT protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Alanine aminotransferase 1 / ALT1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ALT Protein
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Alanine Aminotransferase (ALT) (AA 1-496) protein (GST tag)
ALT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Alanine Aminotransferase (ALT) protein (His tag)ALT Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Alanine Aminotransferase (ALT) (AA 1-496) protein (His tag)ALT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Alanine Aminotransferase (ALT) (AA 2-496) protein (His tag)Crystallography grade ALT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Alanine Aminotransferase (ALT) (AA 2-496) protein (His tag)Crystallography grade ALT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
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: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).
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- Target
- ALT (Alanine Aminotransferase (ALT))
- Alternative Name
- Alanine Aminotransferase 1 (Alt1) (ALT Products)
- Background
- This gene encodes cytosolic alanine aminotransaminase 1 (ALT1) also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009].
- Molecular Weight
- 54.5 kDa
- NCBI Accession
- NP_005300
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