ACER1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714568

This Recombinant ACER1 protein is produced in HEK-293 Cells.

Quick Overview for ACER1 Protein (Myc-DYKDDDDK Tag) (ABIN2714568)

Target: ACER1 (Alkaline Ceramidase 1 (ACER1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This ACER1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Alkaline ceramidase 1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ACER1 (Alkaline Ceramidase 1 (ACER1))

Alternative Name: Alkaline Ceramidase 1

Background: Ceramides are synthesized during epidermal differentiation and accumulate within the interstices of the stratum corneum, where they represent critical components of the epidermal permeability barrier. Excess cellular ceramide can trigger antimitogenic signals and induce apoptosis, and the ceramide metabolites sphingosine and sphingosine-1-phosphate (S1P) are important bioregulatory molecules. Ceramide hydrolysis in the nucleated cell layers regulates keratinocyte proliferation and apoptosis in response to external stress. Ceramide hydrolysis also occurs at the stratum corneum, releasing free sphingoid base that functions as an endogenous antimicrobial agent. ACER1 is highly expressed in epidermis and catalyzes the hydrolysis of very long chain ceramides to generate sphingosine (Houben et al., 2006 [PubMed 16477081] Sun et al., 2008 [PubMed 17713573]).[supplied by OMIM, Jul 2010].

Molecular Weight: 30.9 kDa

NCBI Accession: NP_597999