ALPL Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714570

Recombinant ALPL protein expressed in HEK-293 Cells.

Quick Overview for ALPL Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2714570)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This ALPL protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Alkaline phosphatase / ALPL (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: Alkaline Phosphatase,alpl

Background: This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects.

Molecular Weight: 55.5 kDa

NCBI Accession: NP_000469