ANAPC5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for ANAPC5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2714782)
Target
See all ANAPC5 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This ANAPC5 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human APC5 / ANAPC5 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-740) protein (His tag)
custom-made ANAPC5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-755) protein (His tag)custom-made ANAPC5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 1-755) protein (GST tag)ANAPC5 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Anaphase Promoting Complex Subunit 5 (ANAPC5) (AA 2-232), (partial) protein (GST tag)ANAPC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA, SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))
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Alternative Name
- Apc5,anapc5
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Background
- This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
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Molecular Weight
- 84.9 kDa
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NCBI Accession
- NP_057321
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Pathways
- M Phase
Target
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