MANF Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714930

Recombinant MANF protein expressed in HEK-293 Cells.

Quick Overview for MANF Protein (Myc-DYKDDDDK Tag) (ABIN2714930)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MANF protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human ARMET / ARP protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: Armet,arp

Background: The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related.

Molecular Weight: 18.1 kDa

NCBI Accession: NP_006001