ATP6V1F Protein (Myc-DYKDDDDK Tag)
Quick Overview for ATP6V1F Protein (Myc-DYKDDDDK Tag) (ABIN2715060)
Target
See all ATP6V1F ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ATP6V1F protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ATP6V1F protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F) (AA 1-119) protein (His tag)
custom-made ATP6V1F Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F) (AA 1-119) protein (GST tag)ATP6V1F Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F) (AA 1-119) protein (His-IF2DI Tag)ATP6V1F Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F) (AA 1-119), (full length) protein (GST tag)ATP6V1F Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))
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Alternative Name
- Atp6v1f
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Background
- This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.
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Molecular Weight
- 13.2 kDa
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NCBI Accession
- NP_004222
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Pathways
- Transition Metal Ion Homeostasis, Proton Transport
Target
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