HLCS Protein (Myc-DYKDDDDK Tag)
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- Target See all HLCS Proteins
- HLCS (Biotin-Protein Ligase (HLCS))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HLCS protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Biotin-protein ligase / HLCS protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product HLCS Protein
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Biotin-Protein Ligase (HLCS) (AA 1-726) protein (GST tag)
HLCS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Biotin-Protein Ligase (HLCS) (AA 627-724) protein (GST tag)HLCS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Biotin-Protein Ligase (HLCS) (AA 1-726) protein (Strep Tag)Crystallography grade HLCS Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Biotin-Protein Ligase (HLCS) (AA 1-722) protein (Strep Tag)Crystallography grade HLCS Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- HLCS (Biotin-Protein Ligase (HLCS))
- Alternative Name
- Biotin-Protein Ligase,hlcs (HLCS Products)
- Synonyms
- 410I21.SP6 Protein, D16Jhu34 Protein, HCS Protein, RGD1565360 Protein, holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) Protein, holocarboxylase synthetase Protein, Hlcs Protein, HLCS Protein
- Background
- This gene encodes an enzyme that catalyzes the binding of biotin to carboxylases and histones. The protein plays an important role in gluconeogenesis, fatty acid synthesis and branched chain amino acid catabolism. Defects in this gene are the cause of holocarboxylase synthetase deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Jun 2011].
- Molecular Weight
- 80.6 kDa
- NCBI Accession
- NP_000402
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