DFFA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for DFFA Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2719434)
Target
See all DFFA ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This DFFA protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DFFA / ICAD (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (GST tag)
DFFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA) (AA 1-331) protein (His tag)DFFA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))
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Alternative Name
- Dffa,icad
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Background
- Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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Molecular Weight
- 36.3 kDa
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NCBI Accession
- NP_004392
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Pathways
- Apoptosis, Caspase Cascade in Apoptosis
Target
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