DKK2 Protein (Fc Tag,His tag)
Quick Overview for DKK2 Protein (Fc Tag,His tag) (ABIN2719480)
Target
See all DKK2 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This DKK2 protein is labelled with Fc Tag,His tag.
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Characteristics
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- Recombinant human Dickkopf-2 (DKK2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (Fc Tag)
DKK2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (His tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dickkopf 2 Homolog (DKK2) (AA 34-100) protein (His-IF2DI Tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Dickkopf 2 Homolog (DKK2) (AA 34-259) protein (His-SUMO Tag)DKK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM PB,150 mM NaCl, pH 7.2
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DKK2 (Dickkopf 2 Homolog (DKK2))
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Alternative Name
- Dickkopf-2 (Dkk2)
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Background
- This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6).
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Molecular Weight
- 58.9kD
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NCBI Accession
- NP_055236
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Pathways
- WNT Signaling
Target
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