POLK Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719558

This Recombinant POLK protein is expressed in HEK-293 Cells.

Quick Overview for POLK Protein (Myc-DYKDDDDK Tag) (ABIN2719558)

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This POLK protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DNA polymerase kappa protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: POLK (Polymerase (DNA Directed) kappa (POLK))

Alternative Name: Dna Polymerase kappa

Background: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients.

Molecular Weight: 98.6 kDa

NCBI Accession: NP_057302

Pathways: DNA Damage Repair