DPH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for DPH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2719699)
Target
See all DPH2 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This DPH2 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DPH2 / DPH2L2 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (His tag)
custom-made DPH2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (His tag)custom-made DPH2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (GST tag)DPH2 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489) protein (His tag)DPH2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 85 % SDS
Diphthamide Biosynthesis Protein 2 (DPH2) (AA 1-489), (full length) protein (His-SUMO Tag)DPH2 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))
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Alternative Name
- Dph2,dph2l2
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Background
- This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 51.9 kDa
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NCBI Accession
- NP_001375
Target
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