E2F1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719894

This Recombinant E2F1 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for E2F1 Protein (Myc-DYKDDDDK Tag) (ABIN2719894)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This E2F1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human E2F1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Tarangelo, Lo, Teng, Kim, Le, Watson, Furth, Raman, Ehmer, Viatour: "Recruitment of Pontin/Reptin by E2f1 amplifies E2f transcriptional response during cancer progression." in: Nature communications, Vol. 6, pp. 10028, (2015) (PubMed).

Target Details

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: e2f1

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Molecular Weight: 46.7 kDa

NCBI Accession: NP_005216

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy