ERN1 Protein (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for ERN1 Protein (Myc-DYKDDDDK Tag) (ABIN2720502)
Target
See all ERN1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ERN1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human ERN1 / IRE1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 401-500) protein (GST tag)
ERN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 1-70) protein (GST tag)ERN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 465-977) (Active) protein (GST tag,His tag)ERN1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 80 % as determined by reducing SDS-PAGE. Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Hypothermia and Rewarming Activate a Macroglial Unfolded Protein Response Independent of Hypoxic-Ischemic Brain Injury in Neonatal Piglets." in: Developmental neuroscience, Vol. 38, Issue 4, pp. 277-294, (2016) (PubMed).
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- ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
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Alternative Name
- Ern1,ire1
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Background
- The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
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Molecular Weight
- 109.6 kDa
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NCBI Accession
- NP_001424
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Pathways
- ER-Nucleus Signaling, Unfolded Protein Response
Target
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