EZH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720645

Recombinant EZH2 protein expressed in HEK-293 Cells and has been mentioned in 2+ publications.

Quick Overview for EZH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2720645)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This EZH2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EZH2 / KMT6 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Pedram Fatemi, Salah-Uddin, Modarresi, Khoury, Wahlestedt, Faghihi: "Screening for Small-Molecule Modulators of Long Noncoding RNA-Protein Interactions Using AlphaScreen." in: Journal of biomolecular screening, Vol. 20, Issue 9, pp. 1132-41, (2015) (PubMed).

Tushir, Akbarian: "Chromatin-bound RNA and the neurobiology of psychiatric disease." in: Neuroscience, Vol. 264, pp. 131-41, (2014) (PubMed).

Target Details

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: Ezh2,kmt6

Background: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.

Molecular Weight: 85.8 kDa

NCBI Accession: NP_004447

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation