EZH2 Protein (AA 1-751) (GST tag)

Catalog No. ABIN1353283

Product Details

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-751

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This EZH2 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)

Purpose: EZH2 (Human) Recombinant Protein (P01)

Sequence: MGQTGKKSEK GPVCWRKRVK SEYMRLRQLK RFRRADEVKS MFSSNRQKIL ERTEILNQEW KQRRIQPVHI LTSVSSLRGT RECSVTSDLD FPTQVIPLKT LNAVASVPIM YSWSPLQQNF MVEDETVLHN IPYMGDEVLD QDGTFIEELI KNYDGKVHGD RECGFINDEI FVELVNALGQ YNDDDDDDDG DDPEEREEKQ KDLEDHRDDK ESRPPRKFPS DKIFEAISSM FPDKGTAEEL KEKYKELTEQ QLPGALPPEC TPNIDGPNAK SVQREQSLHS FHTLFCRRCF KYDCFLHRKC NYSFHATPNT YKRKNTETAL DNKPCGPQCY QHLEGAKEFA AALTAERIKT PPKRPGGRRR GRLPNNSSRP STPTINVLES KDTDSDREAG TETGGENNDK EEEEKKDETS SSSEANSRCQ TPIKMKPNIE PPENVEWSGA EASMFRVLIG TYYDNFCAIA RLIGTKTCRQ VYEFRVKESS IIAPAPAEDV DTPPRKKKRK HRLWAAHCRK IQLKKDGSSN HVYNYQPCDH PRQPCDSSCP CVIAQNFCEK FCQCSSECQN RFPGCRCKAQ CNTKQCPCYL AVRECDPDLC LTCGAADHWD SKNVSCKNCS IQRGSKKHLL LAPSDVAGWG IFIKDPVQKN EFISEYCGEI ISQDEADRRG KVYDKYMCSF LFNLNNDFVV DATRKGNKIR FANHSVNPNC YAKVMMVNGD HRIGIFAKRA IQTGEELFFD YRYSQADALK YVGIEREMEI P

Characteristics: Human EZH2 full-length ORF ( NP_004447.2, 1 a.a. - 751 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Guil, Soler, Portela, Carrère, Fonalleras, Gómez, Villanueva, Esteller: "Intronic RNAs mediate EZH2 regulation of epigenetic targets." in: Nature structural & molecular biology, Vol. 19, Issue 7, pp. 664-70, (2012) (PubMed).

Target Details

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2 (EZH2 Products)

Synonyms: ENX-1 Protein, ENX1 Protein, EZH1 Protein, EZH2b Protein, KMT6 Protein, KMT6A Protein, WVS Protein, WVS2 Protein, eya2 Protein, im:7149338 Protein, zgc:136376 Protein, zgc:152758 Protein, EZH2 Protein, enx-1 Protein, enx1 Protein, ezh1 Protein, kmt6 Protein, xez Protein, Enx-1 Protein, Enx1h Protein, mKIAA4065 Protein, ezh2 Protein, olezh2 Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit L homeolog Protein, enhancer of zeste 2 polycomb repressive complex 2 subunit S homeolog Protein, EZH2 Protein, ezh2 Protein, Ezh2 Protein, ezh2.L Protein, ezh2.S Protein

Background: Full Gene Name: enhancer of zeste homolog 2 (Drosophila)
Synonyms: ENX-1,EZH1,KMT6,MGC9169

Gene ID: 2146

NCBI Accession: NM_004456

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation